Tris (hydroxymethyl) aminomethane

Buffer Tris buffer ntabwo ikoreshwa cyane nkumuti wa acide nucleic na proteyine, ariko kandi ifite akamaro gakomeye.Tris yakoreshwaga mu gukura kwa poroteyine mu bihe bitandukanye bya pH.
Imbaraga nkeya ionic ya Tris buffer irashobora gukoreshwa mugukora fibre intera ya lamin muri C. elegans.
Is Tris nayo ni kimwe mu bintu by'ingenzi bigize poroteyine ya electrophoreis.
☑Myongeyeho, Tris nintera yo gutegura surfactants, yihuta yibirunga nibiyobyabwenge.Tris nayo ikoreshwa nkibisanzwe.
RimTrimethylaminomethane ikoreshwa cyane muri acide metabolike ikaze kandi ihumeka.Nibikoresho bya alkaline kandi bifite ingaruka nziza kuri acide metabolike na reaction yibikorwa bya enzyme.
IsTris ikoreshwa kenshi nka biologiya biologiya, kandi agaciro kayo pH ni 6.8, 7.4, 8.0, 8.8.Imiterere yimiterere, pH agaciro gahinduka cyane hamwe nubushyuhe.Muri rusange, agaciro ka pH kagabanuka 0,03 mugihe ubushyuhe bwiyongereye kurwego rumwe.Tris ikoreshwa cyane mugutegura igisubizo cya buffer mubushakashatsi bwibinyabuzima na molekuline ya biologiya.Kurugero, byombi bya TAE na tbe (bikoreshwa muguhagarika aside nucleique) bikunze gukoreshwa mubushakashatsi bwibinyabuzima bikenera Tris.Kuberako irimo amatsinda ya amino, irashobora kwitwara hamwe na aldehydes.
Is Tris ni ishingiro ridakomeye, kandi PKA yayo ni 8.1 ku bushyuhe bwicyumba (25 ℃).Ukurikije inyigisho ya buffer, urwego rwiza rwa Tris buffer ruri hagati ya 7.0 na 9.2.PH agaciro ka Tris ishingiro ryamazi yumuti ni 10.5.Mubisanzwe, hydrochloric aside yongeweho kugirango ihindure agaciro ka pH agaciro kifuzwa kugirango ubone igisubizo cya buffer hamwe nagaciro ka pH.Mugihe kimwe, dukwiye kwitondera ingaruka zubushyuhe kuri pKa ya Tris.Kubera ko Tris buffer ari igisubizo cyoroshye cya alkaline, ADN izatandukana mugisubizo nkiki kugirango irusheho gukomera.Abantu bakunze kongeramo EDTA muri buffer ya Tris hydrochloric kugirango bakore "Te buffer", ikoreshwa muguhindura ADN no kubika.Niba igisubizo cya aside yo guhindura agaciro pH isimbuwe na acide acetike, haboneka "Tae buffer" (Tris / acetate / EDTA), kandi "tbe buffer" (Tris / borate / EDTA) iboneka mugusimbuza aside ya boric .Ubusanzwe ibyo byuma byombi bikoreshwa mubushakashatsi bwa nucleic aside electrophoreis.